HLA B27 Test in Ahmedabad – Flowcytometry and PCR

HLA b27 testHLA-B27 test is used to detect Class I MHC protein in the body which is the genetic marker for spondyloarthritides.

Around 90% of patients with Ankylosing Spondylitis (AS Back pain, 10% false negative) has this test positive in contrast to only 8% of normal people (false positive) will have this test positive.

It is done by two methods in a clinical laboratory : PCR and Flow cytometry.

Difference between the two :

PCR (polymerase chain reaction) : HLA B27 by PCR is the best sensitive/specific test for Ankylosing spondylitis , but little costlier than flow and time it takes 2-3 days in comparison to flow which is available in 1 day.

Flow cytometry HLA B-27 test : In certain cases, there is indeterminate result with flow, which is then to be followed up by PCR test (separate cost)

  1. HLA B27 test by PCR cost in Qline Ahmedabad : Rs 3000, report time 2-3 days
  2. HLA B27 test by Flow cytometer cost : Rs 1800, report time 1 day

Best prices available in Ahmedabad compared to others.

Our findings in this 6 years of HLA Testing we found that : 

  • 0% tests done by PCR gave a inconclusive/indeterminate result.
  • around 6% of tests done by flow cytometry gave an inconclusive/indeterminate result.

How to get the test done?

  • You can visit our Naranpura lab anytime of the day , 24×7 open
  • You can book home visit also, chargeable extra as per distance – around 50-150 rupees depending on distance from main lab.
  • No specific preparation is needed for the HLA-B27 test. Can be done for pregnancy women also.

    You will get email or whatsapp digital report in 1-3 days.

    Other tests which might be prescribed include RA/RF factor test, anti CCP , ESR, CRP, Uric acid which are all done for joint pains.


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